Journal:

Article Title: Circulation of Endemic Type 2 Vaccine-Derived Poliovirus in Egypt from 1983 to 1993

doi: 10.1128/JVI.77.15.8366-8377.2003

Figure Lengend Snippet: Single-step growth curves at 39.5°C in S3 HeLa cells of MEF-1/EGY42 (wild), Qalyubiya/88-1 (cVDPV), Beni Suef/93 (cVDPV), Beni Suef/98 (OPV-like), and Sabin 2.

Article Snippet: Virus titers were determined in plaque assays on HeLa cell monolayer cultures (ATCC CCL-2) incubated at 37°C essentially as described previously ( 51 ).

Techniques:

Journal: Pathogens

Article Title: A Novel Jeilongvirus from Florida, USA, Has a Broad Host Cell Tropism Including Human and Non-Human Primate Cells

doi: 10.3390/pathogens13100831

Figure Lengend Snippet: A549, CV-1, and LLC-MK2 cells inoculated with GRJV1. ( A ) Mock-infected A549 cells, 6 days post-inoculation (dpi). ( B ) Appearance of A549 cells inoculated with GRJV1 6 dpi. Dead cells, perinuclear darkening, and syncytia are evident. ( C ) Mock-infected CV-1 cells, 6 dpi. ( D ) CV-1 cells inoculated with GRJV1 6 dpi. Cell elongation, cytoplasmic vacuoles, dead cells, perinuclear darkening, and syncytia are evident. ( E ) Mock-infected LLC-MK2 cells, 6 dpi. ( F ) LLC-MK2 cells inoculated with GRJV1 6 dpi. Dead cells, intracellular vacuoles, perinuclear darkening, and syncytia are evident. Original images were taken at 400× magnification.

Article Snippet: For this part of the experiment, 50 μL aliquots, each containing 5 × 10 3 PFU of GRJV1, were inoculated onto newly confluent monolayers of A549 ( Homo sapiens [Adenocarcinomic alveolar] cells, ATCC CCL cat. no. 185), BHK-21 ( Mesocricetus auratus [Hamster] kidney cells, CCL cat. no. 10), Caco-2 ( Homo sapiens [Adenocarcinomic colorectal] cells, HTB cat. no. 37), Calu-3 ( Homo sapiens , lung cells HTB Cat. no. 55), CV-1 ( Cercopithecus aethiops [African green monkey] kidney cells CCL cat. no. 70), HeLa ( Homo sapiens cervix cells, CCL cat. no. 2), LLC-MK2 ( Macaca mulatta [Rhesus monkey] kidney cells, CCL cat. no. 7), MDCK ( Canis familiaris [Dog] kidney cells, CCL cat. no. 34), MRC-5 ( Homo sapiens lung cells, CCL cat. no. 171), Neuro-2a ( Mus musculus [Mouse] brain cells, CCL cat. no. 131), OHH1.K ( Odocoileus hemoinus hemionus [Columbian black tail deer] kidney cells, CRL cat. no. 6193), SAEC ( Homo sapiens lung cells, PCS cat. no. 301-010), and Tb1Lu ( Tadarida brasiliensis [Free-tailed bat] lung cells, CCL cat. no. 88) cultured in 3 mL of complete cell growth medium consisting of aDMEM supplemented with 10% heat-inactivated, gamma-irradiated fetal bovine serum (FBS) (GE Healthcare Life Sciences, Pittsburgh, PA, USA, cat. no. SH30070.03IH), GlutaMAX, and PSN.

Techniques: Infection

Journal: Pathogens

Article Title: A Novel Jeilongvirus from Florida, USA, Has a Broad Host Cell Tropism Including Human and Non-Human Primate Cells

doi: 10.3390/pathogens13100831

Figure Lengend Snippet: Viral quantification results.

Article Snippet: For this part of the experiment, 50 μL aliquots, each containing 5 × 10 3 PFU of GRJV1, were inoculated onto newly confluent monolayers of A549 ( Homo sapiens [Adenocarcinomic alveolar] cells, ATCC CCL cat. no. 185), BHK-21 ( Mesocricetus auratus [Hamster] kidney cells, CCL cat. no. 10), Caco-2 ( Homo sapiens [Adenocarcinomic colorectal] cells, HTB cat. no. 37), Calu-3 ( Homo sapiens , lung cells HTB Cat. no. 55), CV-1 ( Cercopithecus aethiops [African green monkey] kidney cells CCL cat. no. 70), HeLa ( Homo sapiens cervix cells, CCL cat. no. 2), LLC-MK2 ( Macaca mulatta [Rhesus monkey] kidney cells, CCL cat. no. 7), MDCK ( Canis familiaris [Dog] kidney cells, CCL cat. no. 34), MRC-5 ( Homo sapiens lung cells, CCL cat. no. 171), Neuro-2a ( Mus musculus [Mouse] brain cells, CCL cat. no. 131), OHH1.K ( Odocoileus hemoinus hemionus [Columbian black tail deer] kidney cells, CRL cat. no. 6193), SAEC ( Homo sapiens lung cells, PCS cat. no. 301-010), and Tb1Lu ( Tadarida brasiliensis [Free-tailed bat] lung cells, CCL cat. no. 88) cultured in 3 mL of complete cell growth medium consisting of aDMEM supplemented with 10% heat-inactivated, gamma-irradiated fetal bovine serum (FBS) (GE Healthcare Life Sciences, Pittsburgh, PA, USA, cat. no. SH30070.03IH), GlutaMAX, and PSN.

Techniques: Virus